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KMID : 1024520090180010015
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Journal of the Environmental Sciences
2009 Volume.18 No. 1 p.15 ~ p.19
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Visualization of Thecal Plates of Lightly Armored Dinoflagellates Cryptoperidiniopsis brodyi and Pfiesteria piscicida (Dinophyceae)
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Park Tae-Gyu
Kang Yang-Sun
Bae Heon-Meen
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Abstract
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Early studies claimed that heterotrophic dinoflagellates Pfiesteria piscicida and related genera may produce a putative water-soluble toxin that causes death of fish and other marine animals. Several methods were tested to visualize plate morphology of Cryptoperidiniopsis brodyi and Pfiesteria piscicida. Cellulose plates of cells were exposed and visualized- by a membrane stripping method using Triton X-100. While calcofluor M2R white stain could readily bind to the thecal plates, details of the plate tabulation were difficult to observe. Fixation with osmium tetroxide produced well preserved cells with little morphological distortion, but thecal plates could not be visualized. Scanning electron microscopy (SEM) observation using the membrane stripping method showed distinctive plate tabulations between C. brodyi and P. piscicida suggesting that this method is a useful tool for morphological identification of lightly armored dinoflagellates.
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KEYWORD
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Cryptoperidiniopsis brodyi, Harmful algal blooms, Membrane stripping method, Plate tabulations, Pfiesteria piscicida, Red tide
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